Table 6.

Increased Il10 mRNA expression was found after each DSS treatment cycle, while Tgfβ was only increased after acute DSS treatment.

Treg-associated transcription factor and cytokines
Foxp3	Proximal colon		Distal colon
	Control mice	DSS-treated mice	Control mice	DSS-treated mice
Cycle 1	0.51 ± 0.15	0.38 ± 0.3	0.70 ± 0.14	0.83 ± 0.07
Cycle 2	1.07 ± 0.1	0.90 ± 0.14	0.80 ± 0.07	0.97 ± 0.09
Cycle 3	1.24 ± 0.35	0.87 ± 0.28	0.72 ± 0.1	1.03 ± 0.11ρ = 0.082
Tgfβ	Proximal colon		Distal colon
	Control mice	DSS-treated mice	Control mice	DSS-treated mice
Cycle 1	0.41 ± 0.05	0.41 ± 0.08	0.61 ± 0.09	1.42 ± 0.19***
Cycle 2	0.50 ± 0.05	0.34 ± 0.04	0.57 ± 0.07	0.80 ± 0.13
Cycle 3	0.68 ± 0.06	0.54 ± 0.09	0.75 ± 0.09	0.84 ± 0.22
Il10	Proximal colon		Distal colon
	Control mice	DSS-treated mice	Control mice	DSS-treated mice
Cycle 1	0.14 ± 0.04	0.18 ± 0.04	0.17 ± 0.05	0.78 ± 0.10**
Cycle 2	0.49 ± 0.06	0.45 ± 0.07	0.53 ± 0.10	1.01 ± 0.16*
Cycle 3	0.58 ± 0.09	0.54 ± 0.13	0.50 ± 0.13	0.90 ± 0.14ρ = 0.076

Colons were collected for examination of the mRNA expression level of Treg transcription factor and associated cytokines. The mRNA expression of Foxp3, Tgfβ and Il10 in the proximal- and distal part of the colon are shown. All results are expressed as + SEM, n = 6. ^* Indicates the significant different between control and DSS-treated mice.

^*P < 0.05.

^**P < 0.01.

^***P < 0.001.