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. 2016 May 12;7:10–19. doi: 10.1016/j.bbrep.2016.05.011

Fig. 1.

Fig. 1.

Gln induces MKP-1 induction in an ERK-dependent way. (A) Lungs were removed at the indicated times after i.v. injection of LPS (50 µg/kg). Gln (750 mg/kg) was given i.p. 5 min after LPS. (B) U0126 (12.5 mg/kg) was injected i.p. 24 h before LPS injection. Lungs were removed at 20 min (C) Gln (750 mg/kg) was given i.p. 5 min after LPS (1 µg or 10 µg/mouse). (D) ERK activity against MBP was performed as described in Materials and Methods. A representative of three to five independent experiments with 3–5 mice/time point/experiment is shown.(E). Gln (40 mM) was added to MHS cells (2×106) 5 min after LPS (1 ng/ml) stimulation. (F) and (G). Gln (20 mM, 40 mM or 80 mM) was added to MHS cells (2×106) 5 min after LPS (1 ng/ml) stimulation. Cells were stimulated for 15 min A representative of three independent experiments is shown.