Fig. 2.

Gln activates Ras/c-Raf (Ser³³⁸)/MEK/ERK pathway in response to LPS. (A) Lungs were removed at the indicated times after i.v. injection of LPS (50 µg/kg). Gln (750 mg/kg) was given i.p. 5 min after LPS. A representative of five independent experiments with 3–5 mice/time point/experiment is shown. (B) Gln (40 mM) was added to MHS cells (2×10⁶) 5 min after LPS (1 ng/ml) stimulation (C) and (D) Serum starved MHS cells were pretreated with FTI (10 µM or 20 µM) for 1 h before stimulation with LPS (1 ng/ml). Gln (40 mM) was added to MHS cells 5 min after LPS. Cells were stimulated for 15 min A representative of three to five independent experiments is shown.