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. 2016 May 12;7:10–19. doi: 10.1016/j.bbrep.2016.05.011

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© 2016 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 7. — Gln and Cys inhibit cPLA₂ phosphorylation, but MKP-1 siRNA abrogates only Gln-induced inhibition.(A) The ears were removed at the indicated time points after DNFB challenge. Gln or Cys was applied three times (×3) in the ear skin after DNFB challenge. A representative of three independent experiments with 3–5 mice/time point/experiment is shown. (B) The ears were removed at 30 min post challenge. (C) LTB₄ level in the ear samples at 24 h after DNFB challenge were calculated as pg of protein/mg of homogenized tissue solution. Data in (C) represent the mean ± standard deviation of three independent experiments with five mice per group. *P<0.05;**P<0.01;***P<0.001.