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. 2016 May 30;7:106–112. doi: 10.1016/j.bbrep.2016.05.020

Fig. 2.

Fig. 2.

Relative levels of 14-3-3 proteins paralogs in untreated and differentiated 3T3-L1 cells 7 days after adipogenic induction. A) 14-3-3 paralogs expression measured by qPCR of differentiated 3T3-L1 cells relative to untreated cells. PPIA was used as normalizing gene. Bars represent averaged mean data from three biological and technical replicates of qPCR. B) 40 µg of whole cell lysate from untreated and differentiated 3T3-L1 cells were loaded per well and separated by SDS/PAGE. Blots were divided for reaction with specific antibodies against each of the different 14-3-3 paralogs and α-Tubulin as loading control. Bars in the graphs represent mean±SD, of values from 3 experiments quantified by densitometry and expressed relative to that of α-Tubulin values from the same western blot line. Asterisks indicate significant differences between samples (using the non-parametric Wilcoxon rank-sum test, p<0.05). C) Same as in B using antibodies against 14-3-3γ and α-Tubulin. Decreasing amounts of whole cell lysate of differentiated cells were loaded per well. B and C) Data were similar in two other experiments.