Table 1.
Primers used for site-specific mutagenesis.
| Primer | Orientation | Nucleotide sequence 5′→3′ |
|---|---|---|
| 16-Haml | + | ccactacgacaatac g |
| 15-Hamr | – | gag caagtcgacgtg |
| 26-E1N5tl | + | ggactgccaatgctcaatctatcc cg |
| 17-E1N6tl | + | tggtcacttacaacagc |
| 23-E1N5br | – | gag cat tggcagtcctgc act gt |
| 17-E1N6br | – | ctgttgtaagtgaacag |
| 17-E1N7tl | + | gaactggtctaaggttc |
| 17-E1N7br | – | aaccttagaagaccaggt cc |
| 24r-Hpsr | – | cagctgcagttacccgtcaacgcc |
| 29-E1N1m | – | ttatgaagtgcgccaagtgtccgg cat at |
| 30-E1N2m | – | aacgactgctcccaatcaagcattgcg tat |
| 27-E1N3m | – | gttcag gag ggtcaaagctcccgttgc |
| 24-E1N4m | – | cgcggccaggcaagccagcgtccc |
| 19 Haml | – | cactacgacaatacgacg t |
| 18 Hamr | – | agcaagtcgacgtgacgt |
| 28-E2N1m | – | tgaat acc caaggcagctggcac at |
| 30-E2N2m | – | tggcacatccaaagt act gccctaaattgc |
| 30-E2N3m | – | gccctaaattgccaagactccctccaa act |
| 30-E2N4m | – | gcacacaagttccaatcgtccgggtgcccg |
| 25-E2N6m | – | tggggg gag caa gag acagacgtg a |
| 30-E2N7m | – | gtgatgctcctccaaaacacgcgtccgcca |
| 30-E2N8m | – | tgtacatggatgcaaagt act gggttc act |
| 27-E2N9m | – | ggggtcggtcaacgc acc ttgaattgc |
| 30-E2N10m | – | tacccctgc act ctccaatttttc cat cat |
| 27-E2N11m | – | gccgcatgccaatgg act cgagga gag cgc |
| E2 for | + | aggtctagaatgttatgattgttttgctac |
| E2 back | + | ct atagtgtcacctaaatccgaaagcttcggcctcagcttgag |