Fig. 1.

Schematic overview over the different plasma preparations. A total of 10 mL of whole blood was used for the preparation of platelet poor plasma (PPP). Two tubes of 10 mL whole blood were handled according to our laboratory protocol for preparation of biobank plasma. Plasma from one of the tubes was frozen for one week, thawed and further processed to eliminate platelet contamination (biobank A, A2, B and B2). As illustrated, after freezing and thawing of the biobank plasma we denote it biobank A. Plasma from the other tube was pipetted from the top of the tube mL by mL to investigate the distribution of platelets in the plasma phase (gradient A–D).