Fig. 2.

Effect of mAbs on levels of endogenous AQP4 in primary cultured astrocytes. (A) Representative immunoblots of lysates treated with 1 µg/ml of C9401 (lane 2), E5415A (lanes 3 and 4), or E5415B (lanes 5 and 6) in the absence (lanes 2, 3, and 5) or presence (lanes 4 and 6) of 500 nM bafilomycin A1 at 37 °C for 24 h using anti-AQP4 (upper panel) or anti-actin (lower panel) antibody. (B) Effect of E5415A or E5415B on levels of endogenous AQP4 (black column) and actin (grey column). Values are determined by Western blotting and estimated as fold relative to non-treated cells (A, lane 1). **(P<0.01), significant difference versus cells treated with C9401 alone; and ## (P<0.01) and # (P<0.05), significant difference between the groups as determined by the Tukey-Kramer method.