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. 2016 Jun 28;7:218–224. doi: 10.1016/j.bbrep.2016.06.018

Fig. 2.

Fig. 2.

Expression and phosphorylation of eEF2K and eEF2 in left ventricles from pressure overload-induced cardiac hypertrophy model mice. Transverse aortic constriction (TAC) or SHAM operation was performed in C57BL/6NJc1 mice. After 3 days from the operation, left ventricles were isolated and immediately frozen. After extraction of protein, total-eEF2K (t-eEF2K, n=8), p-eEF2K at Ser366 (n=8) and p-eEF2 at Thr56 (n=8) were determined by Western blotting. The results were shown as fold increase relative to SHAM. p-eEF2K was normalized to t-eEF2K. t-eEF2K and p-eEF2 were normalized to GAPDH. *p<0.05 vs. SHAM;**p<0.01 vs. SHAM.