Fig. 2.

Effect of concentration of divalent cations on PCK2 activity. (A) Effect of variable Mn²⁺ (25–2000 µM) and 0.01 µM of Mg²⁺. (B) Effect of variable Mn²⁺ (0.5–25 µM) in the presence of fixed Mg²⁺ concentration (2 mM). All reactions were performed in the PEP carboxylation direction as described in Materials and Methods. Mean±SD were adjusted to the Michaelis-Menten equation.