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. 2016 Jun 8;7:124–129. doi: 10.1016/j.bbrep.2016.06.007

Fig. 4.

Fig. 4.

Effect of PCK2 overexpression on glucose and lipid production. (A) Glucose production in HEK293T cells using an empty pCDNA3.1 plasmid as control (14.7±0.5 mg glucose/mg protein) or overexpressing PCK2 (48.1±6.9 mg glucose/mg protein). Shapiro-Wilk test was performed to test normality and a two-sample t-test, to analyze glucose production (*p<0.05). (B) Lipid production in HEK293T cells using an empty pCDNA3.1 plasmid as control in absence (0.9±0.2 absorbance units/mg protein) or presence (1.42±0.1 absorbance units/mg protein) of palmitate or overexpressing PCK2 in presence of palmitate (3.25±0.2 absorbance units/mg protein). Shapiro-Wilk test was performed to test normality. One-way ANOVA following a Tukey post-hoc test was performed to analyze lipid production (*p<0.05, **p<0.01, ***p<0.001).