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Kinetic parameters of human PCK2.
| Substrate | Km(µM) | kcat(s−1) | kcat/Km(M−1 s−1) | Figure |
|---|---|---|---|---|
| OAA decarboxylation: OAA+GTP→PEP+CO2+GDP | ||||
| OAA | 8.7±2.5 | 32.0±2.5 | 3.7×106 | 3A |
| GTP | 29±7 | 19.8±1.5 | 6.9×105 | 3B |
| PEP carboxylation. PEP+GDP+CO2→OAA+GTP | ||||
| PEP | 585±120 | 18.8±1.7 | 3.2×104 | 3C |
| GDP | 8.6±1.0 | 19.1±0.5 | 2.2×105 | 3D |
| HCO3− | 17,454±2529 | 25.3±1.3 | 1.4×103 | 3E |
| Pyruvate formation. OAA+GTP→Pyruvate+CO2+GDP | ||||
| Not detecteda | ||||
| Pyruvate formation. OAA+GDP→Pyruvate+CO2+GDP | ||||
| OAA | 17±2.7 | 2.7±0.1 | 1.6×105 | 3F |
See Section 2.
