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. 2017 Sep 25;16:181. doi: 10.1186/s12944-017-0574-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Effect of SDA on mRNA expression levels of adipogenic transcription factors and genes in 3T3-L1 adipocytes. Two-day post-confluency preadipocytes were incubated with differentiation medium in the presence of SDA (0, 50, and 200 μM). a The expression of C/EBPα, C/EBPβP, PPARγ, and SREBP-1c was evaluated by qRT-PCR with specific primer pairs on Day 3. b The expression of AP2, FAS, SCD-1, LPL, GLUT4, and PEPCK was evaluated by qRT-PCR on Day 6. The relative qRT-PCR values were corrected to 36B4 expression levels and normalized with respect to the control. Data were obtained from three independent experiments and are expressed as mean ± SD; different from control cells: *P < 0.05