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. 2017 Sep 25;10:442. doi: 10.1186/s13071-017-2347-6

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Comparative immunohistochemistry of PKD on rainbow trout liver and spleen during single and co-infection with WD. a Liver section of single Tb infected group, 90 dpe showing presence of few parasite stages in hepatic parenchyma (arrow). b Liver section of Mc-then Tb co-infected group, 120 dp initial exposure to Mc (90 dpe to secondary Tb) showing presence of many parasite stages in hepatic parenchyma and central veins (arrow). c Spleen section of single Tb infected group, 90 dpe showing presence of few parasite stages (arrow). d Spleen section of Mc-then Tb co-infected group, 120 dp initial exposure to Mc (90 dpe to secondary Tb) shows presence of many parasite stages in the parenchyma (arrow) associated with horseshoe-shaped multinucleated giant cell, strong positively IHC stained (inset, arrow). Scale-bars: a, 100 μm (inset 20 μm); b, 100 μm (inset 20 μm); c, 100 μm; d, 100 μm (inset 20 μm)