Table 1.
Comparison of T. bryosalmonae numbers in immunohistochemical stained tissues and histopathological lesion scores in different groups. Presented data are the mean values of T. bryosalmonae numbers from immunohistochemical stained tissues and the results of histopathological lesion scores from kidneys samples at 60, 90 and 120 dpe
| 60 dpe to T. bryosalmonae | 90 dpe to T. bryosalmonae | 120 dpe to T. bryosalmonae | ||||||
|---|---|---|---|---|---|---|---|---|
| Single Tb infected group | Tb-then Mc co-infected group | Mc-then Tb co-infected group | Single Tb infected group | Tb-then Mc co-infected group | Mc-then Tb co-infected group | Single Tb infected group | Tb-then Mc co-infected group | |
| T. bryosalmonae numbers by IHC | ||||||||
| 1-Interstitium | 43.71 ± 21.17 (20–74)b | 39.5 ± 21.67 (24–87)b | 99.4 ± 43.24 (45–164)a | 58.71 ± 30.17 (18–94)b | 68.6 ± 12.09 (55–82)b | 156.0 ± 36.03 (98–192)a | 90.83 ± 21.79 (68–120)a | 121.67 ± 14.57 (110–138)a |
| 2-Blood vessels | 0.71 ± 1.25 (0–3)a | 5.00 ± 10.62 (0–31)a | 5.2 ± 4.15 (0–11)a | 2.86 ± 1.86 (0–5)b | 2.8 ± 1.92 (0–5)b | 8.0 ± 4.0 (2–12)a | 3.83 ± 1.83 (2–7)a | 3.33 ± 1.53 (2–5)a |
| 3-Interepithelial | 0.43 ± 0.79 (0–2)a | 0.5 ± 1.07 (0–3)a | 0.6 ± 0.89 (0–2)a | 0.0 (0–0)b | 0.0 (0–0)b | 2.2 ± 1.3 (0–3)a | 0.0 (0–0)a | 0.0 (0–0)a |
| Histopathological lesions | ||||||||
| A-Interstitium | ||||||||
| 1-Infiltration of inflammatory cells | 3 (2–4) | 3 (2–5) | 4 (3–5) | 4 (4–5) | 5 (4–5) | 6 (4–6) | 5 (5–6) | 6 (5–6) |
| 2-Fibosis | 3 (2–5) | 3 (2–5) | 4 (3–5) | 4 (4–5) | 4 (3–5) | 6 (4–6) | 5 (4–6) | 6 (5–6) |
| 3-Haemorrhage | 1 (1–2) | 3 (2–4) | 4 (3–5) | 3 (3–4) | 4 (4–5) | 3 (2–3) | 2 (2–3) | 2 (2–3) |
| 4-Parasites with daughter cells | 3 (2–5) | 3 (2–4) | 4 (4–5) | 4 (3–5) | 4 (4–5) | 5 (4–6) | 5 (4–6) | 5 (5–6) |
| B-Blood vessels | ||||||||
| 1-Thrombi | 1 (1–2) | 2 (1–3) | 3 (2–3) | 2 (1–4) | 3 (2–3) | 3 (2–4) | 3 (2–4) | 2 (2–3) |
| 2-Parasites | 1 (1–2) | 1 (0–4) | 2 (0–2) | 1 (0–2) | 1 (0–2) | 2 (1–2) | 1 (1–2) | 1 (1–2) |
| C-Renal tubules | ||||||||
| 1-Tubulonephrosis & necrosis | 3 (2–4) | 3 (3–4) | 3 (2–4) | 2 (1–2) | 2 (2–3) | 3 (2–3) | 0 (0–1) | 0 (0–1) |
| 2-Nephron neogenesis | 1 (1–2) | 1 (0–1) | 2 (1–3) | 3 (2–4) | 3 (2–4) | 4 (4–5) | 5 (5–6) | 5 (5–6) |
| 3-Intraluminal stages of parasite | 0 (0–0) | 0 (0–0) | 0 (0–0) | 0 (0–0) | 0 (0–0) | 0 (0–0) | 0 (0–0) | 0 (0–0) |
Numbers in parentheses indicate the lowest and highest values counted in 10 microscopic fields (400×), based on the following scoring criteria: 0, no lesions; 1, minimal lesions; 2, mild lesions; 3, mild to moderate lesions; 4, moderate lesions; 5, moderate to severe lesions; 6, severe lesions. Different letters a, b in the same raw for each time point separately were significant at P < 0.05