Fig. 3.

Effect of cobalt ions on neutrophil chemotaxis. A. Filters stained with haematoxylin showing migrated neutrophils at ×40 magnification. B. Migrated neutrophils were counted at ×40 magnification. Neutrophil migration was significantly increased in response to 0.75 mM CoCl₂ and 100 ng/ml LPS (24 h) conditioned media from MonoMac 6 cells compared to untreated controls. 1 µg/ml CLI-095 significantly abrogated the observed CoCl₂-mediated chemotaxis (P<0.001). LPS-induced neutrophil chemotaxis was also inhibited by CLI-095 (P<0.001). Data is representative of three independent experiments each using a different neutrophil donor. ***=P<0.001, Student's t-test.