Fig. 3.

Viral-assisted spectral tracing of mouse cerebellum. a Multi-color neurons, labeled with a two-component viral delivery system. Two viral vectors carrying ssAAV-PHP.eB:TET-DIO-XFP (XFP = mNeonGreen, mTurquiose2, or mRuby) and ssAAV-PHP.eB:ihSyn-tTA-WPRE were injected into the retro-orbital sinus of an adult mouse. After 3 weeks of expression the animals were sacrificed and the brain was cut into 300 μm thick sections. The section was optically cleared in refractive index matching solution (RIMS) and then imaged using a confocal microscope. Refer to [55] for details. b Using the 3-D volume data, five Purkinje cells were manually traced in NeuTube and their traces overlaid on the 3-D volume. c Traces of the five Purkinje cells from b