Fig. 2.

Determination of optimal temperatures of PDC and AHAS activities of recombinant TmAHAS. The reaction mixtures were equilibrated at each temperature for 4 min before adding the enzyme. The relative activities of 100% equals to highest measured specific activity for both enzymes at 85 °C (18.6 U mg⁻¹ for PDC and 235 U mg⁻¹ for AHAS activity). The open symbols indicate the PDC and the filled symbols indicate the AHAS activity. Each point is an average of experimental values in duplicates.