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Apparent kinetic parameters for PDC and AHAS activities of recombinant TmAHAS.
| Substrate | AHAS activitya |
PDC activity |
||||
|---|---|---|---|---|---|---|
| Apparent Km (µM) | Apparent Vmax (U mg−1) | kcat (S−1) | Apparent Km (µM) | Apparent Vmax (U mg−1) | kcat (S−1) | |
| Pyruvate | 16,400±1900 | 246±7 | 99 | ND | ND | ND |
| TPP | 57±6.0 | 242±4 | 97 | 35±0.6 | 25.9±0.6 | 8 |
| FAD | 0.15±0.07 | 134±11 | 54 | 0.12±0.05 | 18.1±1.7 | 7 |
ND, not determined as the saturation curve was not following the Michaelis-Menten kinetics.
Kinetic parameters are from [10].
