Fig. S2.

The HC11/4ICD cell line was treated with scrambled (Control) or STAT5 siRNA (STAT5 KD) for 24 h. as indicated in Fig. 4 and then treated with vehicle or 5 μg/ml of prolactin for 48 h. RNA was extracted and analyzed for β-casein and WAP expression by quantitative RT-PCR as described in Fig. 4. Results indicate that as predicted STAT5 knockdown resulted in suppressed prolactin stimulation of β-casein and WAP when compared to the scrambled control.