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. 2017 Sep 25;18:754. doi: 10.1186/s12864-017-4053-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — SNPs in sugarcane miRNAs and qRT-PCR validation. a Reads mapping of variant sof-miR5564b-3p and sof-miRN396-3p showed SNPs in their sequences. b Venn diagram of target genes of normal and variant sof-miRN396 showed because of the SNP (C - > T) in the “seed” region sof-miRN396 lost 11 target genes (e.g. LRKS5, WEX, Y2242) but gained new target genes (POL2, E139, Y5162). c qRT-PCR validation for candidate miRNAs (sof-miR159-3p, sof-miR166a-3p, sof-miR396a-5p and sof-miR5564b-3p) and variant miRNA (sof-miR5564bV-3p)