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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Stroke. 2017 Mar 1;48(4):1033–1043. doi: 10.1161/STROKEAHA.116.015609

Figure 2. Inhibitors capable of preventing hemin-induced death also inhibit hemoglobin-induced-cell death.

Figure 2

(A) Primary neurons were exposed to 1.5μM hemoglobin (LD50) and ferroptotic and necroptotic inhibitors. Values reflect mean ± SD at representative concentration in brackets. Grayscale coding reflects a continuum from no protection from hemoglobin toxicity by a designated chemical inhibitor (black) to maximal possible cell viability (white). (B) Concentrations-responses of protective inhibitors in hemoglobin toxicity. Values represent mean ± SD, except for U0126 where medians are given. * p<0.05 versus hemoglobin, # p< 0.05 versus inactive analogs U0124 or Necrostatin-1i. (C) Representative live/dead staining are shown. Scale bar = 50μm.