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. 2016 Aug 20;8:120–126. doi: 10.1016/j.bbrep.2016.08.017

Fig. 3.

Fig. 3.

Snail1 expression in colon cancer cells increases their motility and invasiveness but additional N-cadherin expression does not increase these activities. (A) Migration assay to determine motility of Snail+ DLD-1 cells and Snail+ DLD-1 cells expressing N-cadherin (Snail+Ncad cells) compared with control DsRed+ DLD-1 cells. Values represent the mean±S.E.; n=at least 3 times. (B) Invasion assay using Matrigel to determine invasiveness of Snail+ DLD-1 cells and Snail+DLD-1 cells expressing N-cadherin (Snail+Ncad cells) compared with DsRed+ DLD-1 cells. Values represent the mean±S.E.; n=at least 3 times. (C) Immunoblot detection of N-cadherin in stable N-cadherin transfectants of Snail+ DLD-1 cells. Vinculin was used as a loading control. (D) Invasion assay using Matrigel to determine invasiveness of Snail+ SW480 cells compared with control (transfected with an empty vector) SW480 cells. Values represent the mean±S.E.; n=at least 3 times. *p<0.01 compared with control (DsRed). **p<0.05 compared with Snail1. ***p<0.01 compared with control (empty vector).