Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Aug 20;8:127–138. doi: 10.1016/j.bbrep.2016.08.016

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Published by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Fig. 3. — Factors affecting ATPase activity Effect of MgCl₂ on ATPase activity of GcsT (A) isotopic assay, (B) NADH coupled assay. The results were plotted as % relative activity versus MgCl₂ concentration. Optimum activity was found at 8 mM MgCl_2.for GcsT. (C-D) Effect of divalent cations on ATPase activity. Extent of hydrolysis was plotted as percentage activity as a function of individual divalent cations Mg²⁺, Mn²⁺, Co²⁺, Cd²⁺, Cs²⁺, Zn²⁺ and Ca²⁺. Mg²⁺ show maximum increase in ATPase activity. Inset shows radiographs having Gcs with the same divalent cations. ATPase activity in presence of different concentrations (0.25–20 mM) of (E) Mn²⁺ and (F) Co²⁺. The results were plotted as % relative activity versus divalent concentration. Three independent experiments were performed and results were averaged for relative activity calculation.