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Substrate binding of LdGcs. (A) Thermogram of binding of LdGcs, left, middle and right panel corresponds to ATP, L-Glutamate and L-Cysteine. 15 μM recombinant protein in HEPES pH 7.5 was titrated against 200 μM of substrate. Kd values were obtained by fitting the data using Origin 7.0 software. (B) Tryptophan fluorescence saturation binding isotherms of Gcs.
