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Km and Vmax values were determined by the Michaelis-Menten equation. The lineweaver burk plot was also considered for determining these paramerters.
| Kmapp(mM) | Vmaxapp(uM/min/mg) | |
|---|---|---|
| ATP | 6.25±.4 | 85.32 |
| L-Glutamate | 9.2±1.1 | 108.26 |
| L-Cysteine | 1.7±0.15 | 75.18 |
