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Apparent Kd values of ATP, L-Glutamate and L-Cysteine for different mutants of LdGcs. Kd values were calculated as mentioned in materials and methods.
| Constructs | Kd Value (μM) | ||
|---|---|---|---|
| ATP | L-Glutamate | L-Cysteine | |
| GcsF | 2.7± 0.3 | 93.7± 1.8 | 177± 2.6 |
| E55A | 2.5±1 | 793±.182 | 95±2.1 |
| E92A | 3235±.937 | 107±.16 | 115±.35 |
| R498A | 1550±238 | 890±.167 | 164±13 |
| E99A | 5.8±.3 | 666±.3 | 173±13 |
