| STANDARD ACSF SOLUTION (5X) FOR PERFUSION | |||
| (Stock solution) | |||
| Compound | MW | Final Conc. (mM) | Amount for 2 L 5X |
| NaCl | 58.44 | 126 | 73.6 |
| NaHCO3 | 84.01 | 24 | 20.2 |
| glucose | 180.2 | 10 | 18 |
| KCl | 74.55 | ♦ 4.5 | 3.355 |
| MgSO4 | 120.4 | 2 | 2.41 |
| NaH2PO4 | 120 | 1.25 | 1.5 |
| Ascorbate | 176.1 | 0.4 | 0.705 |
| CaCl2.2H2O [1 M] stock | 147 | 2 | 2 mL / L * |
| * add 2 mL CaCl2 [1 M] for 1 L aCSF (1x) oxygenated solution | |||
| pH = 7.4 when oxygenated, Osm 310 - 320 | |||
| ♦ A slightly elevated [K+]o is used for this aCSF solution (compared to normal aCSF 2.5 mM KCl) to increase excitability of hippocampal networks and facilitate the emergence of theta oscillations. |
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