Fig. 4.

The generation and longevity of serum IgY levels specific for the individual components of a prototype poultry red mite recombinant antigen cocktail vaccine and native protein preparations during field trial evaluation.

The specific IgY levels of individual hens from the Vaccine (left panels) and Placebo (right panels) groups in trial 2 to the individual recombinant vaccine antigens: Deg-SRP-1, Deg-PUF-1, Deg-VIT-1 and the native protein mite extracts: soluble mite extract (SME) and integral membrane mite extract (IMME) were quantified using ELISAs. The antigen-specific IgY levels (represented by A_490nm) of 8 individual hens randomly sampled from each group at 9 time points, are indicated with black diamonds (♦) and the mean response with the solid black line. The timings of the primary and secondary vaccinations (V1 and V2) and of the mite challenge (Ch) are indicated with the arrows on x-axis. The mite population data of Vaccine and Placebo groups are overlaid (broken black lines), with the error bars giving the S.E.M.