Figure 5.

INEPT spectra indicate high mobility of the methylation N-helix in the CF in functional arrays. Black dots represent the methylation sites and dashed lines represent a portion of the HAMP region contained in the CF used in this study. Top: sliding a 50-residue window from N- to C-terminus of the CF sequence identifies the most probable mobile segment (red block, H267-Q316), corresponding to the N-helix side of the methylation region. No other contiguous 50-residue segment contains all of the amino acid residues observed in the INEPT spectra. Bottom: sliding a pair of 25-residue windows sequences adjacent in the structure along the length of the helix hairpin (within outlined red boxes) does not identify any positions at which the pair contain all of the amino acid residues observed in the INEPT spectra.