Figure 8.

¹H–¹³C INEPT of the kinase-on (blue, CF4Q) and kinase-off (red, CF4Q.A411V) states of the uniformly ¹³C, ¹⁵N-labeled CF incorporated into vesicle-mediated functional complexes with CheA and CheW. Spectra are corrected for the difference in nmol of CF. The same resonances are observed for both signaling states, but the kinase-off state exhibits greater intensity for all peaks except peaks corresponding only to the C-terminal tail (Phe and Pro). Inset shows overlay of the 1D projection of each spectrum. Same NMR conditions as Figure 3.