Table 3. Peak Volumesa in 1H–13C and 1H–15N INEPT Spectra of 13C15NCF4Q and 13C15NCF4QΔ34 Assembled with CheA and CheW into Functional Native-Like Arrays on Vesiclesb.
| residue/s | ω1/1H | ω2/13C or 15N | CF4Q volume | CF4QΔ34 volume | mobile region residues | CF4Q – CF4QΔ34 = Δ34 volume | Δ34 (tail) residues |
|---|---|---|---|---|---|---|---|
| total Cα (no Gly) | 82.8 | 47.6 | 48 | 35.2 | 34 | ||
| Arg Nε | 7.3 | 84.9 | 5.7 | 2.8 | 3 | 2.9 | 3 |
| Asn Cβ + Tyr Cβ | 2.6 | 39.1 | 4.1 | 2.4 | 1 Asn, 1 Tyr | 1.7 | 2 Asn |
| Glu Cγ | 2.2 | 36.3 | 7.1 | 3.7 | 5 | 3.4 | 3 |
| Gly Cα | 4.0 | 45.4 | 2.8 | 2.8 | 3 | 0.0 | 0 |
| Gly N | 8.3, 8.2 | 108.7, 107.4 | 2.5 | 2.2 | 3 | 0.3 | 0 |
| His Cβ | 3.1 | 29.7 | 0.9 | 0.7 | 1 | 0.2 | 0 |
| His Cε1 | 7.8 | 138.4 | 0.9 | 0.8 | 1 | 0.1 | 0 |
| Ile Cβ | 1.8 | 38.7 | 3.2 | 1.9 | 2 | 1.3 | 1 |
| Ile Cδ1 | 0.8 | 13.0 | 3.3 | 2.0 | 2 | 1.3 | 1 |
| Leu Cβ | 1.6 | 42.2 | 4.3 | 2.5 | 3 | 1.7 | 2 |
| Met Cβ | 2.0 | 17.1 | 1.5 | 1.5 | 1 | 0.0 | 0 |
| Phe | 7.2 | 131.8 | 1.1 | 0 | 0 | 1.1 | 1 |
| Pro Cδ | 3.7 | 50.7 | 1.3 | 0 | 0 | 1.3 | 7 |
| Thr Cβ | 4.2 | 69.8 | 8.4 | 5.4 | 6 | 3.0 | 3 |
| Trp Nε | 10.0 | 129.1 | 1.0 | 0 | 0 | 1.0 | 1 |
| Tyr Cδ | 7.1 | 133.4 | 1.1 | 1.0 | 1 | 0.1 | 0 |
a
All peak volumes are corrected for differences in nmol of CF in the sample. HC peak volumes are then calibrated based on Tyr Cδ = 2 in the CF4QΔ34 vesicle sample, and then normalized to the number of unresolved correlations contributing to the peak (5 for Phe and 2 for Tyr Cδ) so that the reported volume should correspond to the number of residues. HN peak volumes are calibrated based on Trp Nε = 1 in the CF4Q vesicle sample.
b
Bold columns compare peak volumes to residues of the most probable 50-residue mobile region; italicized columns compare difference volumes to residues of the deleted 34-residue tail.