Figure 3.

Genetic inactivation of Wise rescued palate morphogenesis in Pax9^del/del mice. (A, E, I) Whole mount view of the palate in WT, Pax9^del/del, and Pax9^del/del;Wise^-/- pups at P0. (B–D, F–H, J–L) Representative frontal sections from anterior, middle, and posterior regions of the secondary palate in E16.5 WT, Pax9^del/del, and Pax9^del/del;Wise^-/- embryos. Of 20 Pax9^del/del;Wise^-/- pups examined after E16.5, 14 exhibited fused palate, whereas the other 6 Pax9^del/del;Wise^-/- pups had cleft palate. Asterisk indicates the cleft palate in panels E–H. Arrows point to the molar tooth germs. E, embryonic day; P0, postnatal day; p, palatal shelf; t, tongue; WT, wild type.