Figure 5.

Inactivation of Wise rescued palatal shelf elevation/reorientation in Pax9^del/del embryos. (A–I) Representative frontal sections from anterior, middle, and posterior regions of the secondary palate in E14.5 WT, Pax9^del/del, and Pax9^del/del;Wise^-/- embryos. Arrows point to the molar tooth germs. (J–L) Representative frontal sections from the posterior region of the palatal shelves of E13.5 WT, Pax9^del/del, and Pax9^del/del;Wise^-/- embryos showing fluorescent staining of hyaluronic acid (red color). White dashed line marks the proximal boundary of the palatal shelf area used for quantification of fluorescence intensity. (M) Quantification of mean fluorescence intensity of hyaluronic acid staining in the WT, Pax9^del/del, and Pax9^del/del;Wise^-/- samples (n = 4 for each genotype). Fluorescence intensity of hyaluronic acid staining was normalized against the area of palatal shelves. Error bars represent SD. p, palatal shelf; t, tongue; WT, wild type. *P < 0.05. **P < 0.01.