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. 2017 Sep 26;5:17044. doi: 10.1038/boneres.2017.44

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Copyright © 2017 The Author(s)

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Overexpression of AFF1 impairs osteogenic differentiation. (a) Real-time RT-PCR shows that the mRNA level of AFF1 is significantly increased. n=3. ***P<0.001. (b) Western blot analysis of AFF1. (c) Representative images of ALP staining. Overexpression of AFF1 decreases the intensity of the staining. (d) Quantitative analyses of ALP activity. n=5. *P<0.05. (e) Representative images of ARS staining of MSCs. (f) Quantitative analyses of calcium mineralization. n=5. *P<0.001. (g–i) Real-time RT-PCR reveals decreased mRNA expression of RUNX2, SP7 and BGLAP. n=3. *P<0.05, **P<0.01 and ***P<0.001.