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. 2017 Sep 26;5:17044. doi: 10.1038/boneres.2017.44

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Copyright © 2017 The Author(s)

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Depletion of AFF4 decreases osteogenic differentiation. (a) Real-time RT-PCR shows successful knockdown of AFF4. n=3. ***P<0.001. (b) Western blot analysis of AFF4. (c) Depletion of AFF4 reduces the proliferation of MSCs. n=5. *P<0.05 and ***P<0.001 (d) Representative images of ALP staining. Depletion of AFF4 decreases the intensity of the staining. (e) Quantitative analyses of ALP activity. n=5. *P<0.05. (f) Representative images of ARS staining of MSCs. (g) Quantitative analyses of calcium mineralization. n=5. **P<0.01. (h–j) Real-time RT-PCR reveals reduced mRNA expression of SP7 and BGLAP. n=3. *P<0.05 and **P<0.01.