Skip to main content
. 2016 Aug 26;8:139–145. doi: 10.1016/j.bbrep.2016.08.021

Fig. 2.

Fig. 2.

Expression of IL-17 after discontinuation of cyclosporin A (CsA). (A) Protocol for sampling of serum, spleen and spinal cord after discontinuation of CsA. (B) Levels of IL-17A, IL-17F, or IL-17A/F heterodimer in serum. Serum on day 30 or 40 was collected and used for IL-17A, IL-17F, or IL-17A/F heterodimer measurement by ELISA in triplicate wells and is shown as the mean±SEM. (C) Analysis of gene expression of IL-17A or IL-17F in spleen or spinal cord on days 30 or 40. Total RNA extracted from the spleen or spinal cord of individual mice on days 30 or 40 was subjected to quantitative real-time PCR. Expression levels are normalized to G3PDH. (D) At day 30 of EAE, spleen cells and CNS infiltrating cells were evaluated for secretion of IL-17 and for expression of Foxp3 by CD4+ cells. Error bars represent mean±SEM (B–C, n=6; D, n=5–7 per group). *P<0.05. CFA, complete Freund's adjuvant; i.v., intravenous; i.p., intraperitoneally.