Fig. 4.

Postulated signal transduction pathways of ARA-S modulation of cytoskeleton via CB1, CB2, TRPV1 receptors. Possible mechanisms of receptor-mediated activation involve different routes including both Rho/ROCK kinase and PI3/Akt kinase pathways resulting in cytoskeletal alterations. Inhibition of Rho/ROCK kinase by H1152 decreases ROCK and ROCK-mediated inhibition of PI3K. Inhibition of PI3/Akt kinase by LY294002 completely inhibits Akt formation and effects on cytoskeleton. Akt effects on cytoskeleton are also inhibited by treatment with L-NAME, inhibitor of e-NOS activity.