Table 1.
Characterization of the effects of (A) the antagonists of the CB1, CB2 and vanilloid receptors and (B) inhibitor of Rho kinase and PI3 on phosphorylation of protein kinases in ARA-S-stimulated HBEC.
| (A) | |||||
|---|---|---|---|---|---|
| Control | ARA-S | ARA-S+CB1antagonist | ARA-S+CB2 antagonist | ARA-S+vanilloid antagonist | |
| Akt | 1 | 2.30±0.12 | 1.72±0.18 | 1.71±0.19 | 1.51±0.18 |
| MAPK | 1 | 2.33±0.16 | 1.78±0.20 | 1.57±0.32 | 1.53±0.21 |
| c-JUN | 1 | 1.49±0.07 | 1.22±0.12 | 0.97±0.09 | 0.72±0.18 |
| JNK | 1 | 2.00±0.12 | 1.47±0.10 | 1.31±0.18 | 0.96±0.31 |
| (B) | |||||
|---|---|---|---|---|---|
| Control | ARA-S | ARA-S+LY294002 | ARA-S+H1152 | ARA-S+LY294002+ H1152 | |
| Akt | 1 | 2.32±0.15 | 0.30±1.00 | 3.73±0.35 | 0.57±0.36 |
| MAPK | 1 | 2.28±0.19 | 1.73±0.19 | 1.10±0.10 | 0.26±0.05 |
| c-JUN | 1 | 1.58±0.10 | 0.69±0.14 | 1.19±0.10 | 1.25±0.02 |
| JNK | 1 | 2.02±0.17 | 1.10±0.10 | 0.95±0.13 | 0.57±0.13 |
Data indicates density analysis of Western blots. Values (ratio relative to control value) were analyzed using ANOVA followed by Bonferroni pairwise comparisons. (A) Treatment with the CB1, CB2 or vanilloid receptor antagonists causes a statistically significant reduction in phosphorylation of all four kinases relative to the ARA-S- stimulated value (p<0.05). (B) ARA-S phosphorylated value vs. ARA-S with LY294002, H1152, or LY294002+H1152 were statistically different for Akt, MAPK, c-JUN, and JNK (p<0.05 in all cases). LY294002 and H1152 inhibit ARA-S-stimulated phosphorylation of MAPK, c-JUN and JNK. LY294002 also inhibits the phosphorylation of Akt, while H1152 enhances the ARA-S-induced phosphorylation of Akt.