Fig. 5.

mRNA and protein levels of KATs in the mouse liver and kidney. The mRNA transcripts of mouse kat1 and kat3 in the liver and kidney were analyzed using both RT-PCR and qRT-PCR. All final products in RT-PCR experiments were analyzed by 1% agarose gel electrophoresis (A). Relative expression levels of kat1 and kat3 genes were shown as percentages of gapdh gene expression in qRT-PCR tests (B). The specificity of the purified mouse KAT3 antibody was tested by Western blotting. Both recombinant mouse KAT1 and KAT3 proteins were run in a SDS-PAGE, transferred to a PVDF membrane and immuno-stained with the purified mouse KAT3 antibody. The antibody recognized the recombinant mouse KAT3 protein very well, without cross-reaction with recombinant mouse KAT1 (C). Using the purified mouse KAT3 antibody, KAT3 protein was detected both in liver and kidney, but a more intensive band was seen in the kidney (D).