Table 1.
Kinetic parameters of mKAT 1 & 3 towards glutamine and phenylpyruvate.
| Km (mM) | kcat (min−1) | kcat/Km (min−1 mM−1) | ||
|---|---|---|---|---|
| mKAT I/GTK | Glutamine | 2.3±0.4 | 410.5±23.7 | 178.5 |
| Phenylpyruvate | 0.8±0.3 | 300.2±16.3 | 375.3 | |
| mKAT3/GTL | Glutamine | 0.7±0.3 | 160.0±15.0 | 228.6 |
| Phenylpyruvate | 0.6±0.2 | 190.0±28.3 | 316.7 |
The activities were measured as described in the Method section. The Km and kcat were derived by using varying concentrations (0.2–20 mM) of individual substrate in the presence of 20 mM of glutamine or 5 mM phenylpyruvate. The parameters were calculated by fitting the Michaelis–Menten equation to the experimental data using the enzyme kinetics module. Results are means±SE.