Fig. 1.

Tissue wide mRNA transcripts accumulation of RNase4 and RNase5 in cattle. A total of 1 µg of DNase-I-treated RNA from the indicated tissues was reverse transcribed into cDNA. The resulting cDNA preparation was diluted 1/10 and 2 µL of that cDNA was subjected to qPCR to analyse the accumulation of the mRNA transcripts of bovine RNase4 and RNase5. The accumulation of RNase4 and RNase5 mRNA transcripts is represented relative to the geometric mean of the mRNA transcript accumulation of three reference genes, i.e. beta-tubulin, GAPDH, and cyclophilin A. Data in panels A and B show the mean (±SE) from analysis of tissue from three individual cattle, while panels C and D show triplicate analyses of one individual cow.