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. 2016 Sep 23;8:261–267. doi: 10.1016/j.bbrep.2016.09.001

Fig. 2.

Fig. 2.

Assessment of cross-reactivity of RNase4 and RNase5 polyclonal antibodies. Cross-reactivity of RNase4 and RNase5 polyclonal antibodies was evaluated by western blotting. Varying amounts of purified RNase4, RNase5 and bovine pancreatic RNaseA (as indicated) were subjected to SDS-PAGE and then transferred onto a nitrocellulose membrane. This was probed with affinity purified primary anti-RNase4 or anti-RNase5 antibodies and subsequently with HRP conjugated goat anti-rabbit IgG antibody. The signals were visualized by enhanced chemiluminescence followed by exposure to X-ray film for 30 s.