Fig. 3.

Abundance of RNase4 and RNase5 protein in the indicated tissues in cattle. (A). Abundance of RNase4 (upper two panels) and RNase5 (lower two panels) in pancreas, spleen, endometrium, kidney, salivary gland, lungs, liver, lymph node, small intestine, skin, and mammary gland tissues of a cow as evaluated by western blot. A total of 105 µg of total protein was subjected to SDS-PAGE and then transferred onto a nitrocellulose membrane. This was probed with affinity purified primary anti-RNase4 or anti-RNase5 antibodies and subsequently with HRP conjugated goat anti-rabbit IgG antibody. The signals were visualized by enhanced chemiluminescence followed by exposure to X-ray film for 3 min (B). Ponceau S stained images of the membranes is shown to indicate the protein loaded in Fig. 3A.