Sample | Condition | Effector (E) : Target (T) |
PBMCs | 1 - positive control for NK cell cytotoxicity | 50 : 1 + IL-2 |
2 | 50 : 1 | |
3 | 25 : 1 | |
4 | 12.5 : 1 | |
5 | 6.25 : 1 | |
6 | T only | |
7 - negative control for K562 death | E only | |
8 - positive control for K562 death | T + Tween | |
NK cells (purified) | 1 - positive control for NK cell cytotoxicity | 5 : 1 + IL-2 |
2 | 5 : 1 | |
3 | 2.5 : 1 | |
4 | 1.25 : 1 | |
5 | 0.625 : 1 | |
6 | T only | |
7 - negative control for K562 death | E only | |
8 - positive control for K562 death | T + Tween |