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. 2017 Aug 9;(126):56191. doi: 10.3791/56191
Sample Condition Effector (E) : Target (T)
PBMCs 1 - positive control for NK cell cytotoxicity 50 : 1 + IL-2
2 50 : 1
3 25 : 1
4 12.5 : 1
5 6.25 : 1
6 T only
7 - negative control for K562 death E only
8 - positive control for K562 death T + Tween
NK cells (purified) 1 - positive control for NK cell cytotoxicity 5 : 1 + IL-2
2 5 : 1
3 2.5 : 1
4 1.25 : 1
5 0.625 : 1
6 T only
7 - negative control for K562 death E only
8 - positive control for K562 death T + Tween