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. Author manuscript; available in PMC: 2017 Sep 26.
Published in final edited form as: Cell Rep. 2017 Apr 4;19(1):101–113. doi: 10.1016/j.celrep.2017.03.038

Figure 2. Macrophages Suppress Genotoxic Stress and Cancer Cell Death.

Figure 2

TS1 tumor cells (TCs) were cultured with Taxol and assayed by flow cytometry at the indicated time points.

(A–D) Flow cytometric analysis of DNA content (n = 4 independent experiments) (A), phospho-H3 immunoreactivity (n = 3 independent experiments) (B), γH2AX immunoreactivity (n = 3 independent experiments) (C), and phospho-p53 immunoreactivity (n = 3 independent experiments) of the TS1 TC line in response to Taxol treatment (50 nM), and the effects of co-culture with bone marrow-derived macrophages (BMDMs) (D).

(E) Comparison of the effects of co-culture with MHCII+ BMDMs on Taxol-induced accumulation of tumor cells with sub-2C DNA content and γH2AX immunoreactivity (n = 4 independent experiments).

(F) Comparison of the effects of direct cell co-culture versus BMDM-conditioned media (CM) supplemented daily [denoted BMDM-CM(+)] on Taxol-induced accumulation of tumor cells with sub-2C DNA content and γH2AX immunoreactivity (n = 4 independent experiments).

(G) Quantification of caspase activation in response to Taxol treatment and the effects of BMDM-CM as measured by western blotting. Representative western blots are shown on the left, and quantification of each caspase is shown on the right (n = 3 independent experiments).

All data are presented as mean ± SEM. Student’s t test was used to assess significance between treatment groups: *p < 0.05; **p < 0.01; ***p < 0.001. See also Figures S2 and S3.