Fig. 2.

15d-PGJ₂ and doxorubicin induced apoptosis synergistically in Caki-2 cells. Capase-3: Caki-2 cells were treated with 1 μM doxorubicin in the absence or presence of 20 μM 15d-PGJ₂. Caspase-3 activities were determined as initial velocities (A). Data are expressed as means±SE. (n=3). ^*P<0.05, compared with control. ##P < 0.01, compared with 15d-PGJ₂ alone. PI staining: Caki-2 was treated with 5 μM doxorubicin in the absence or presence of 20 μM 15d-PGJ₂ for 24 h. PI-stained Caki-2 cells were photographed (B) and counted (C). Scale bar=100 µm. Cell death is expressed as % (PI-stained cell numbers/total cell numbers). Data are expressed as means±SE. (n=12). Since PI-stained cells were not detected in the control culture, significant difference compared with the control culture could not be tested.