Fig. 1.

CRAT expression and the acylcarnitine profile in human myotubes. (A) The protein abundance of CRAT (70 kDa) was determined by western blotting using VDAC1 (31 kDa) as loading control. Band intensities were quantified by densitometry and expressed as the ratio between CRAT and VDAC band intensities. (B) The mRNA level of CRAT was determined by qPCR and normalized to TBP levels. (C) Acylcarnitine species in human myotubes obtained from obese persons with and without T2DM and lean controls were analysed by LC-MS. Mean±SEM is shown, n =9. Statistical analysis was assessed by One-way ANOVA with Dunnett's multiple comparisons test, ^*(p<0.05).