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. 2017 Sep 26;6:e28652. doi: 10.7554/eLife.28652

Figure 3. Serial passaging of Influenza A/Wuhan/1995 H3N2.

(A) Serial passaging workflow in modified proteostasis environments in MDCKHSF1 cells. (B) Serial passaging workflow in control MDCKYFP cells. (C) Hemagglutination titers at intermittent passages for each folding environment; error bars represent SEM for biological triplicates. (D) Dn/Ds ratios for each viral population, normalized to the ratio of non-synonymous sites to synonymous sites in the influenza genome (3.5). Figure 3—figure supplement 1. Multiplicity of infection and hemagglutination titers during serial passaging.

Figure 3.

Figure 3—figure supplement 1. Multiplicity of infection and hemagglutination titers during serial passaging.

Figure 3—figure supplement 1.

(A) Multiplicity of infection at intermittent passages during serial passaging, determined via a TCID50 assay. (B) Hemagglutination titers for each passage of serial passaging. Error bars represent SEM between biological triplicates.