Fig. 3.

Effect of EGCG, EGC and GA on neurite outgrowth of human SH-SY5Y neuroblastoma cells. A cell suspension (2.5×10⁴ cells/well) was plated in a 24-well plate. EGCG, EGC and GA dissolved in 0.01% DMSO were added to the culture medium to make a final concentration of 0.05 µM and cultured for 72 h at 37 °C. Photos of control cells (a), and cells treated with EGC (b), GA (c), EGC+GA (d), or EGCG (e). Neurite length (f) and neurite number (g) of cells treated with catechins. Scale bar is 50 µm. Each value represents the mean±SEM. Asterisks and # represent significant differences with the control (*) and with EGC+GA (#) (p<0.05, Bonferroni's t-test).